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- Title
A Study for Testing the Antifungal Susceptibility of Yeast by the Japanese Society for Medical Mycology (JSMM) Method - The Proposal of the Modified JSMM Method 2009.
- Authors
Nishiyama, Yayoi; Abe, Michiko; Ikeda, Reiko; Uno, Jun; Oguri, Toyoko; Shibuya, Kazutoshi; Maesaki, Shigefumi; Mohri, Shinobu; Yamada, Tsuyoshi; Ishibashi, Hiroko; Hasumi, Yayoi; Abe, Shigeru
- Abstract
The Japanese Society for Medical Mycology (JSMM) method used for testing the antifungal susceptibility of yeast. the MIC end point for azole antifungal agents, is currently set at IC80. It was recently shown, however that there is an inconsistency in the MIC value between the JSMM method and the CLSI M27-A2 (CLSI) method, in which the end-point was to read as IC50. To resolve this discrepancy and reassess the JSMM method, the MIC for three azoles, fluconazole, itraconazole and voriconazole were compared to 5 strains of each of the following Candida species: C. albicans, C. glabrata, C. tropicalis, C. parapsilosis and C. krusei, for a total of 25 comparisons, using the JSMM method, a modified JSMM method, and the CLSI method. The results showed that when the MIC end- point criterion of the JSMM method was changed from IC80 to IC50 (the modified JSMM method), the MIC value was consistent and compatible with the CLSI method. Finally, it should be emphasized that the JSMM method, using a spectrophotometer for MIC measurement, was superior in both stability and reproducibility, as compared to the CLSI method in which growth was assessed by visual observation.
- Subjects
ANTIFUNGAL agents; ANTI-infective agents; FUNGICIDES; YEAST; AZOLES; SPECTROPHOTOMETRY; POLYENE antibiotics
- Publication
Japanese Journal of Medical Mycology, 2010, Vol 51, Issue 3, p153
- ISSN
0916-4804
- Publication type
Article
- DOI
10.3314/jjmm.51.153