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- Title
Telomere length regulates TERRA levels through increased trimethylation of telomeric H3K9 and HP1?
- Authors
Arnoult, Nausica; Van Beneden, Amandine; Decottignies, Anabelle
- Abstract
Gene silencing by the repressive telomeric chromatin environment, referred to as telomere position effect (TPE), has been well characterized in yeast and depends on telomere length. However, proof of its existence at native human chromosome ends has remained elusive, mainly owing to the paucity of genes near telomeres. The discovery of TERRAs, the telomeric noncoding RNAs transcribed from subtelomeric promoters, paved the way to probing for telomere-length impact on physiological TPE. Using cell lines of various origins, we show that telomere elongation consistently represses TERRA expression. Repression is mediated by increased trimethylated H3K9 density at telomeres and by heterochromatin protein HP1?, with no detectable spreading of the marks beyond the telomeric tract, restricting human TPE to telomere transcription. Our data further support the existence of a negative-feedback mechanism in which longer TERRA molecules repress their own transcription upon telomere elongation.
- Subjects
GENE silencing; TELOMERES; HUMAN chromosomes; HETEROCHROMATIN; GENETIC transcription
- Publication
Nature Structural & Molecular Biology, 2012, Vol 19, Issue 9, p948
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.2364