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- Title
Characterization of mycobacterial triacylglycerols and monomeromycolyl diacylglycerols from Mycobacterium smegmatis biofilm by electrospray ionization multiple-stage and high-resolution mass spectrometry.
- Authors
Purdy, Georgiana; Pacheco, Sophia; Turk, John; Hsu, Fong-Fu
- Abstract
The storage of triacylglycerols (TAGs) is essential for non-replicating persistence relevant to survival and the re-growth of mycobacteria during their exit from non-replicating state stress conditions. However, the detailed structures of this lipid family in mycobacteria largely remain unexplored. In this contribution, we describe a multiple-stage linear ion-trap mass spectrometric approach with high resolution mass spectrometry toward direct structural analysis of the TAGs, including a novel lipid subclass previously defined as monomeromycolyl diacylglycerol (MMDAG) isolated from biofilm of Mycobacterium smegmatis, a rapidly growing, non-pathogenic mycobacterium that has been used as a tool for molecular analysis of mycobacteria. Our results demonstrate that the major isomer in each of the molecular species of TAGs and MMDAGs consists of the common structure in which Δ18:1- and 16:0-fatty acyl substituents are exclusively located at sn-1 and sn-2, respectively. Several isomers were found for most of the molecular species, and thus hundreds of structures are present in this lipid family. More importantly, this study revealed the structures of MMDAG, a novel subclass of TAG that has not been previously reported by direct mass spectrometric approaches.
- Subjects
MYCOBACTERIA; TRIGLYCERIDES; DIGLYCERIDES; ELECTROSPRAY ionization mass spectrometry; MYCOBACTERIUM smegmatis; BIOFILMS; MASS spectrometry
- Publication
Analytical & Bioanalytical Chemistry, 2013, Vol 405, Issue 23, p7415
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-013-7179-4