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- Title
Expression of a bi-functional and thermostable amylopullulanase in transgenic rice seeds leads to autohydrolysis and altered composition of starch.
- Authors
Chiang, Chih-Ming; Yeh, Feng-Shi; Huang, Li-Fen; Tseng, Tung-Hi; Chung, Mei-Chu; Wang, Chang-Sheng; Lur, Hu-Shen; Shaw, Jei-Fu; Yu, Su-May
- Abstract
Overexpression of bacterial-derived starch metabolic enzymes in plant starch storage organs represents a valuable strategy for improving starch quality, bioprocessing and nutritional value. Transgenic rice seeds producing a thermostable and bifunctional starch hydrolase, amylopullulanase (APU) fromThermoanaerobacter ethanolicus39E, were generated. Starch in these seeds could be hydrolyzed with optimal temperatures between 85 and 95 °C, which resulted in complete conversion of starch into soluble sugars and production of protein-enriched flour within a few hours. By expressing various levels of APU, rice seeds containing reduced amounts of amylose, which is an important factor affecting starch quality, were obtained without a significant impact on grain yield. Elevation in granule-bound pullulanase activity correlates with the reduction of amylose in developing APU-containing rice seeds. APU was found to be localized within amyloplasts and in cell walls, which could be the result of overexpression of APU with a signal peptide. This study establishes novel approaches to alter starch properties, accelerate bioprocessing of starch and production of protein-enriched flour from rice seeds, and could significantly impact the industrial and food uses of cereals.
- Subjects
GENE expression in plants; TRANSGENIC rice; PLANT gene mapping; PLANT genetics; RICE; STARCH
- Publication
Molecular Breeding, 2005, Vol 15, Issue 2, p125
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-004-3919-7