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- Title
Enantioselective synthesis of ( S)-2-cyano-2-methylpentanoic acid by nitrilase.
- Authors
Yoshida, Toyokazu; Mitsukura, Koichi; Mizutani, Takuya; Nakashima, Ryo; Shimizu, Yasuyo; Kawabata, Hiroshi; Nagasawa, Toru
- Abstract
The nitrilase gene of Rhodococcus rhodochrous J1 was expressed in Escherichia coli using the expression vector, pKK223-3. The recombinant E. coli JM109 cells hydrolyzed enantioselectively 2-methyl-2-propylmalononitrile to form ( S)-2-cyano-2-methylpentanoic acid (CMPA) with 96 % e.e. Under optimized conditions, 80 g ( S)-CMPA l was produced with a molar yield of 97 % at 30 °C after a 24 h without any by-products.
- Subjects
NITRILASES; RHODOCOCCUS rhodochrous; RECOMBINANT DNA; ENANTIOSELECTIVE catalysis; GENETIC vectors
- Publication
Biotechnology Letters, 2013, Vol 35, Issue 5, p685
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-012-1131-0