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- Title
Type 1 ribosome-inactivating proteins from Phytolacca dioica L. leaves: differential seasonal and age expression, and cellular localization.
- Authors
Parente, Augusto; Conforto, Barbara; Maro, Antimo; Chambery, Angela; Luca, Paolo; Bolognesi, Andrea; Iriti, Marcello; Faoro, Franco
- Abstract
The expression of type 1 ribosome-inactivating proteins (RIPs) in Phytolacca dioica L. leaves was investigated. Fully expanded leaves of young P. dioica plants (up to 3 years old) expressed two novel RIPs, dioicin 1 and dioicin 2. The former was also found in developing leaves from adult P. dioica within about two and a half weeks after leaf development, and the latter continuously synthesized, with no seasonal or ontogenetic constraint. Fully expanded leaves from adult P. dioica expressed four RIPs (PD-Ls1–4) exhibiting seasonal variation. RIPs were localized in the extracellular space, in the vacuole and in the Golgi apparatus of mesophyll cells . Dioicin 1 and dioicin 2 showed rRNA N-β-glycosidase activity and displayed the following properties, respectively: (1) Mr values of 30,047.00 and 29,910.00, (2) pIs of 8.74 and 9.37, and (3) IC50 values of 19.74 (0.658 nM) and 6.85 ng/mL (0.229 nM). Furthermore, they showed adenine polynucleotide glycosylase activity and nicked pBR322 dsDNA. The amino acid sequence of dioicin 2 had 266 amino acid residues, and the highest percentage identity (81.6%) and similarity (84.6%) with PAP-II from Phytolacca americana, while its identity with other RIPs from Phytolaccaceae was around 40%.
- Subjects
OMBU; PHYTOLACCACEAE; PHYTOLACCA; RIBOSOMES; MASS spectrometry; RNA; AMINO acid sequence
- Publication
Planta: An International Journal of Plant Biology, 2008, Vol 228, Issue 6, p963
- ISSN
0032-0935
- Publication type
Article
- DOI
10.1007/s00425-008-0796-z