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- Title
Confirmatory Analysis of Nitroimidazoles and Hydroxy Metabolites in Honey by Dispersive-Solid Phase Extraction and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry.
- Authors
Li, Xiaowei; Ke, Yuebin; Wang, Yingyu; Wang, Chengfei; Ye, Dongyang; Hu, Xue; Zhou, Lan; Xia, Xi
- Abstract
An ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry (UHPLC-MS/MS) method was developed and validated for confirmatory analysis of four nitroimidazoles and three hydroxy metabolites in honey. Honey samples were dissolved in 2% formic acid solution and nitroimidazoles and metabolites were isolated and enriched by dispersive-solid phase extraction using mixed-mode strong cation-exchange sorbent. The determination involves separation of analytes on an UHPLC C18 column and detection by multiple reaction monitoring in positive ionization mode. The recovery of the method was ranged from 90.2 to 105.6% with inter-day relative standard deviations of less than 11.2%. The limits of detection and limits of quantification were in the ranges of 0.02–0.07 µg/kg and 0.05–0.2 µg/kg, respectively. Honey samples from the market were analyzed to demonstrate the applicability of the proposed method.
- Subjects
NITROIMIDAZOLES; METABOLITE analysis; HONEY analysis; LIQUID chromatography-mass spectrometry; SOLID phase extraction; CONFIRMATORY factor analysis; TANDEM mass spectrometry
- Publication
Molecules, 2018, Vol 23, Issue 12, p3350
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules23123350