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- Title
Establishment and characterization of a cytotrophoblast cell line from normal placenta of human origin.
- Authors
Rong-Hao, Li; Luo, Shu-yi; Zhuang, Lin-Zhi; Rong-Hao, L; Luo, S; Zhuang, L Z
- Abstract
A cell line has been established from human placentae at the first trimester of normal pregnancy. The cell line was obtained by culture of purified cytotrophoblast cells in serum-free medium supplemented with epidermal growth factor, insulin, dexamethasone and 0.1% bovine serum albumin. The cells can be subcultured for >30 passages in one to three splits. All the cells were mononuclear epithelial-like cells positive to cytokeratin 18, gonadotrophin-releasing . hormone (GnRH), neuropeptide Y, neurotensin, leucine-enkephalin, dopamine and 5-hydroxytryptamine inununo-cytochemical staining. The cells secreted GnRH, progesterone and oestradiol (in the presence of testosterone) but little human chorionic gonadotrophin and no β-endorphin. The cell line showed human karyotypes and had a population doubling time of 48 h in serum-free medium. However, the cells would stop growing in the medium containing fetal bovine serum. A normal cytotrophoblast cell line established in serum-free medium will be particularly useful in the study of cytotrophoblast cell proliferation and differentiation.
- Publication
Human Reproduction, 1996, Vol 11, Issue 6, p1328
- ISSN
0268-1161
- Publication type
journal article
- DOI
10.1093/oxfordjournals.humrep.a019381