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- Title
In Vitro Fermentation Characteristics and Fiber-Degrading Enzyme Kinetics of Cellulose, Arabinoxylan, β -Glucan and Glucomannan by Pig Fecal Microbiota.
- Authors
Bai, Yu; Zhou, Xingjian; Li, Na; Zhao, Jinbiao; Ye, Hao; Zhang, Shiyi; Yang, Hongjian; Pi, Yu; Tao, Shiyu; Han, Dandan; Zhang, Shuai; Wang, Junjun; Inoue, Ryo
- Abstract
Non-digestible polysaccharides are of great significance to human and animal intestinal health. Cellulose, arabinoxylan, β−glucan and glucomannan were selected in the present study to investigate the fermentation characteristics and fiber-degrading enzyme kinetics by inoculating pig fecal microbiota in vitro. Our results showed that fermentation of arabinoxylan and β-glucan produced the highest amount of acetate and lactate, respectively. The abundance of Prevotella_9 was the highest in β-glucan group and positively correlated with lactate and acetate. Glucomannan fermentation produced the highest amount of butyrate, and the abundance of Lachnospiraceae_XPB_1014_group and Bacteroides were the lowest. A significant negative correlation was found between Lachnospiraceae_XPB_1014_group, Bacteroides and butyrate. Exo-β-1,4-xylanase had the highest activity at 24 h during arabinoxylan fermentation. The activity of β-glucosidase and β-mannosidase at 36 h were higher than those at 15 h in the glucomannan group. The abundance of Prevotella_9 was positively correlated with β-glucosidase while Lachnospiraceae_XPB_1014_group and Bacteroides were negatively correlated with β-xylosidase. Our findings demonstrated the β-glucan and arabinoxylan promote proliferation of Prevotella_9, with the preference to secret β-glucosidase, β-mannosidase and the potential to produce lactate and acetate. Butyrate production can be improved by inhibiting the proliferation of Lachnospiraceae_XPB_1014_group and Bacteroides, which have the lack of potential to secret β-xylosidase.
- Subjects
ENZYME kinetics; BETA-glucans; BUTYRATES; GLUCOMANNAN; FERMENTATION; ARABINOXYLANS; CELLULOSE; SWINE
- Publication
Microorganisms, 2021, Vol 9, Issue 5, p1071
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms9051071