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- Title
The putative polysaccharide deacetylase Ba0331: cloning, expression, crystallization and structure determination.
- Authors
Andreou, Athena; Giastas, Petros; Arnaouteli, Sofia; Tzanodaskalaki, Mary; Tzartos, Socrates J.; Bethanis, Kostas; Bouriotis, Vassilis; Eliopoulos, Elias E.
- Abstract
Ba0331 is a putative polysaccharide deacetylase from Bacillus anthracis, the etiological agent of the disease anthrax, that contributes to adaptation of the bacterium under extreme conditions and to maintenance of the cell shape. In the present study, the crystal structure of Ba0331 was determined at 2.6 Å resolution. The structure consists of two domains: a fibronectin type 3‐like (Fn3‐like) domain and a NodB catalytic domain. The latter is present in all carbohydrate esterase family 4 enzymes, while a comparative analysis of the Fn3‐like domain revealed structural plasticity despite the retention of the conserved Fn3‐like domain characteristics. The crystal structure of Ba0331, a putative polysaccharide deacetylase from Bacillus anthracis, was determined. The structure comprises two domains: a fibronectin type 3‐like domain and a NodB catalytic domain.
- Subjects
POLYSACCHARIDES; DEACETYLASES; BACILLUS anthracis
- Publication
Acta Crystallographica: Section F, Structural Biology Communications, 2019, Vol 75, Issue 4, p312
- ISSN
2053-230X
- Publication type
Article
- DOI
10.1107/S2053230X19001766