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- Title
PEG matrix enables cell-mediated local BMP-2 gene delivery and increased bone formation in a porcine critical size defect model of craniofacial bone regeneration.
- Authors
Wehrhan, Falk; Amann, Kerstin; Molenberg, Aart; Lutz, Rainer; Neukam, Friedrich W.; Schlegel, Karl A.
- Abstract
Purpose This study addressed the suitability of a polyethylene glycol ( PEG) matrix as scaffold for cell-mediated local BMP-2 gene transfer in a calvarial critical size defect ( CSD) model. Materials and methods PEG matrix (degradation time 10 days) and PEG membrane (degradation time 120 days) were used in the pig calvarial model. Cylindrical (1 × 1 cm) CSD (9 per animal; 20 animals) were filled with: (i) HA/ TCP, covered by PEG membrane (group 1); (ii) HA/ TCP, mixed with PEG matrix (group 2); and (iii) HA/ TCP mixed with BMP-2 transfected osteoblasts and PEG matrix (group 3). BMP-2/4 gene transfer: liposomal in vitro transfection of BMP-2/ V5-tag fusion-protein. Quantitative histomorphometry (toluidine blue staining) after 2, 4 and 12 weeks assessed bone formation. Semiquantitative immunohistochemistry estimated the expression of BMP-2 and V5-tag. Results Group 3 showed significantly higher new bone formation than groups 1, 2 at 4 ( P < 0.05) and 12 ( P < 0.02) weeks. BMP-2- V5-tag was detected for 4 weeks. BMP-2 expression in group 3 was higher compared to all other groups after 2 and 4 ( P < 0.02) weeks. Conclusions The PEG matrix serves as scaffold for cell-mediated BMP-2 gene delivery in guided bone regeneration facilitating cell survival and protein synthesis for at least 4 weeks. Local BMP-2 gene delivery by PEG matrix-embedded cells leads to increased bone formation during critical size defect regeneration.
- Subjects
BONE regeneration; GENETIC transformation; BONE morphogenetic proteins; POLYETHYLENE glycol; SKULL diseases; IMMUNOHISTOCHEMISTRY; GENE expression
- Publication
Clinical Oral Implants Research, 2012, Vol 23, Issue 7, p805
- ISSN
0905-7161
- Publication type
Article
- DOI
10.1111/j.1600-0501.2011.02223.x