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- Title
OEPR Cloning: an Efficient and Seamless Cloning Strategy for Large- and Multi-Fragments.
- Authors
Liu, Chang-Jun; Jiang, Hui; Wu, Lei; Zhu, Ling-Yun; Meng, Er; Zhang, Dong-Yi
- Abstract
Here, an efficient cloning strategy for large DNA fragments and for simultaneous assembly of multiple DNA fragments assembly is presented. This strategy is named OEPR (based on Overlap Extension PCR and Recombination in vivo). OEPR cloning is a seamless, restriction- and ligation-independent method. The method takes advantage of both homologous recombination enzymes in E. coli and overlap PCR. Using OEPR cloning, a long fragment (1-6 kb) or multiple fragments (2-4 fragments) can be easily constructed and simultaneously assembled into a target vector.
- Publication
Scientific Reports, 2017, p44648
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep44648