We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Stability and catalytic properties of lipase immobilized on chitosan encapsulated magnetic nanoparticles cross-linked with genipin and glutaraldehyde.
- Authors
Liu, Yun; Zhou, Hua; Wang, Liuyang; Wang, Shihui
- Abstract
BACKGROUND Among many cross-linkers for enzyme immobilization, genipin (Gen) and glutaraldehyde (Glu) as cross-linkers have been commonly investigated to improve the catalytic performance of enzyme immobilized on chitosan encapsulated magnetic nanoparticles ( CMNPs). In this work, we compared the stability and catalytic properties of lipase Candida rugosa ( CRL) immobilized on CMNPs cross-linked with Gen and Glu, respectively. The CMNPs were first characterized by TEM, XRD and FT-IR. Moreover, some crucial parameters affecting catalytic performances were optimized for Gen- CMNPs-CRL and Glu- CMNPs-CRL preparation. RESULTS The Gen- CMNPs-CRL showed maximum activity at pH 8.0 and 40°C, and retained more than 95% of its initial activity after 7 days storage at 25°C. After 5 cycles re-usage, Gen- CMNPs-CRL still retained over 80% of its initial activity, while Glu- CMNPs-CRL retained only 26% of its initial activity. Kinetic studies confirmed that Gen- CMNPs-CRL and Glu- CMNPs-CRL presented higher substrate affinity characteristics ( Km) than free CRL. FT-IR analysis showed that the variance of β-sheet element in the secondary structure of CRL might contribute to the stability and activity enhancement of Gen- CMNPs-CRL. CONCLUSIONS Gen- CMNPs-CRL showed higher pH, temperature, storage and operational stabilities than Glu- CMNPs-CRL. Thus, genepin is a better promising cross-linker than glutaraldehyde for lipase immobilization on CMNPs. © 2015 Society of Chemical Industry
- Subjects
MAGNETIC nanoparticles; LIPASES; CHITOSAN; CATALYTIC activity; GLUTARALDEHYDE; GARDENIA; CANDIDA; AGLYCONE derivatives
- Publication
Journal of Chemical Technology & Biotechnology, 2016, Vol 91, Issue 5, p1359
- ISSN
0268-2575
- Publication type
Article
- DOI
10.1002/jctb.4732