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- Title
Identifying Genes Involved in Alkaloid Biosynthesis in Vinca minor through Transcriptomics and Gene Co-Expression Analysis.
- Authors
Stander, Emily Amor; Sepúlveda, Liuda Johana; Dugé de Bernonville, Thomas; Carqueijeiro, Inês; Koudounas, Konstantinos; Lemos Cruz, Pamela; Besseau, Sébastien; Lanoue, Arnaud; Papon, Nicolas; Giglioli-Guivarc'h, Nathalie; Dirks, Ron; O'Connor, Sarah Ellen; Atehortùa, Lucia; Oudin, Audrey; Courdavault, Vincent
- Abstract
The lesser periwinkle Vinca minor accumulates numerous monoterpene indole alkaloids (MIAs) including the vasodilator vincamine. While the biosynthetic pathway of MIAs has been largely elucidated in other Apocynaceae such as Catharanthus roseus, the counterpart in V. minor remains mostly unknown, especially for reactions leading to MIAs specific to this plant. As a consequence, we generated a comprehensive V. minor transcriptome elaborated from eight distinct samples including roots, old and young leaves exposed to low or high light exposure conditions. This optimized resource exhibits an improved completeness compared to already published ones. Through homology-based searches using C. roseus genes as bait, we predicted candidate genes for all common steps of the MIA pathway as illustrated by the cloning of a tabersonine/vincadifformine 16-O-methyltransferase (Vm16OMT) isoform. The functional validation of this enzyme revealed its capacity of methylating 16-hydroxylated derivatives of tabersonine, vincadifformine and lochnericine with a Km 0.94 ± 0.06 µM for 16-hydroxytabersonine. Furthermore, by combining expression of fusions with yellow fluorescent proteins and interaction assays, we established that Vm16OMT is located in the cytosol and forms homodimers. Finally, a gene co-expression network was performed to identify candidate genes of the missing V. minor biosynthetic steps to guide MIA pathway elucidation.
- Subjects
INDOLE alkaloids; BIOSYNTHESIS; ALKALOIDS; CATHARANTHUS roseus; FLUORESCENT proteins
- Publication
Biomolecules (2218-273X), 2020, Vol 10, Issue 12, p1595
- ISSN
2218-273X
- Publication type
Article
- DOI
10.3390/biom10121595