We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Transcriptome Profile Reveals Differences between Remote and Ischemic Myocardium after Acute Myocardial Infarction in a Swine Model.
- Authors
Pulido, María; de Pedro, María Ángeles; Álvarez, Verónica; Marchena, Ana María; Blanco-Blázquez, Virginia; Báez-Díaz, Claudia; Crisóstomo, Verónica; Casado, Javier G.; Sánchez-Margallo, Francisco Miguel; López, Esther
- Abstract
Simple Summary: Understanding the molecular basis of acute myocardial infarction is necessary to identify new therapeutic targets. In this work, a comparative transcriptome analysis was performed to identify differences in the expression profile between infarcted and remote areas of the myocardium in a porcine model of acute myocardial infarction. Acute myocardial infarction (AMI) is the consequence of an acute interruption of myocardial blood flow delimiting an area with ischemic necrosis. The loss of cardiomyocytes initiates cardiac remodeling in the myocardium, leading to molecular changes in an attempt to recover myocardial function. The purpose of this study was to unravel the differences in the molecular profile between ischemic and remote myocardium after AMI in an experimental model. To mimic human myocardial infarction, healthy pigs were subjected to occlusion of the mid-left anterior descending coronary artery, and myocardial tissue was collected from ischemic and remote zones for omics techniques. Comparative transcriptome analysis of both areas was accurately validated by proteomic analysis, resulting in mitochondrion-related biological processes being the most impaired mechanisms in the infarcted area. Moreover, Immune system process-related genes were up-regulated in the remote tissue, mainly due to the increase of neutrophil migration in this area. These results provide valuable information regarding differentially expressed genes and their biological functions between ischemic and remote myocardium after AMI, which could be useful for establishing therapeutic targets for the development of new treatments.
- Subjects
MYOCARDIAL infarction; MYOCARDIUM; TRANSCRIPTOMES; SWINE; BLOOD flow; CORONARY vasospasm
- Publication
Biology (2079-7737), 2023, Vol 12, Issue 3, p340
- ISSN
2079-7737
- Publication type
Article
- DOI
10.3390/biology12030340