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- Title
VaAPRT3 Gene is Associated With Sex Determination in Vitis amurensis.
- Authors
Men, Yan; Li, Ji-Rui; Shen, Hai-Lin; Yang, Yi-Ming; Fan, Shu-Tian; Li, Kun; Guo, Yin-Shan; Lin, Hong; Liu, Zhen-Dong; Guo, Xiu-Wu
- Abstract
In the past decade, progress has been made in sex determination mechanism in Vitis. However, genes responsible for sexual differentiation and its mechanism in V. amurensis remain unknown. Here, we identify a sex determination candidate gene coding adenine phosphoribosyl transferase 3 (VaAPRT3) in V. amurensis. Cloning and sequencing of the VaAPRT3 gene allowed us to develop a molecular marker able to discriminate female individuals from males or hermaphrodites based on a 22-bp InDel. Gene expression and endogenous cytokinin content analysis revealed that the VaAPRT3 gene is involved in sex determination or, to be precise, in female organ differentiation, through regulating cytokinin metabolism in V. amurensis. This study enlarged the understanding of sex determination mechanism in the genus Vitis , and the sex marker could be used as a helpful tool for sexual identification in breeding programs as well as in investi g ation and collection of V. amurensis germplasms.
- Subjects
SEX determination; GRAPES; SEX differentiation (Embryology); GENE expression; GENETIC code
- Publication
Frontiers in Genetics, 2021, Vol 12, p1
- ISSN
1664-8021
- Publication type
Article
- DOI
10.3389/fgene.2021.727260