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- Title
A reporter system for replication-competent gammaretroviruses: the inGluc-MLV-DERSE assay.
- Authors
Aloia, A L; Duffy, L; Pak, V; Lee, K E; Sanchez-Martinez, S; Derse, D; Heidecker, G; Cornetta, K; Rein, A
- Abstract
Although novel retroviral vectors for use in gene-therapy products are reducing the potential for formation of replication-competent retrovirus (RCR), it remains crucial to screen products for RCR for both research and clinical purposes. For clinical-grade gammaretrovirus-based vectors, RCR screening is achieved by an extended S+L− or marker-rescue assay, whereas standard methods for replication-competent lentivirus detection are still in development. In this report, we describe a rapid and sensitive method for replication-competent gammaretrovirus detection. We used this assay to detect three members of the gammaretrovirus family and compared the sensitivity of our assay with well-established methods for retrovirus detection, including the extended S+L− assay. Results presented here demonstrate that this assay should be useful for gene-therapy product testing.
- Subjects
RETROVIRUSES; GENE therapy; VIRAL replication; DETECTION of microorganisms; LENTIVIRUSES; SENSITIVITY analysis
- Publication
Gene Therapy, 2013, Vol 20, Issue 2, p169
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/gt.2012.18