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- Title
Investigation of the bystander effect in CHO-K1 cells.
- Authors
Kaźmierczak, Urszula; Banaś, Dariusz; Braziewicz, Janusz; Buraczewska, Iwona; Czub, Joanna; Jaskóła, Marian; Kaźmierczak, Łukasz; Korman, Andrzej; Kruszewski, Marcin; Lankoff, Anna; Lisowska, Halina; Nesteruk, Marta; Szefliński, Zygmunt; Wojewódzka, Maria
- Abstract
Abstract: Aim: Investigation of the bystander effect in Chinese Hamster Ovary cells (CHO-K1) co-cultured with cells irradiated in the dose range of 0.1–4Gy of high LET 12C ions and X-rays. Background: The radiobiological effects of charged heavy particles on a cellular or molecular level are of fundamental importance in the field of biomedical applications, especially in hadron therapy and space radiation biology. Materials and methods: A heavy ion 12C beam from the Heavy Ion Laboratory of the University of Warsaw (HIL) was used to irradiate CHO-K1 cells. Cells were seeded in Petri dishes specially designed for irradiation purposes. Immediately after irradiation, cells were transferred into transwell culture insert dishes to enable co-culture of irradiated and non-irradiated cells. Cells from the membrane and well shared the medium but could not touch each other. To study bystander effects, a clonogenic survival assay was performed. Results: The survival fraction of cells co-cultured with cells irradiated with 12C ions and X-rays was not reduced. Conclusions: The bystander effect was not observed in these studies.
- Publication
Reports of Practical Oncology & Radiotherapy, 2014, Vol 19, pS37
- ISSN
1507-1367
- Publication type
Article
- DOI
10.1016/j.rpor.2014.04.015