We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cichorioside a biocoumarin modulates lipid and glucose storage on 3T3-L1 cell lines: In vitro and in silico approach.
- Authors
Alqahtani, Shahad Mohammed Nasser; Alsuliman, Dalya Ramzi; Alasoom, Abdullah Jalal; Burshed, Hussein Ali; Alshawush, Marwan Mohamed; Altaweel, Abdullah Abdulhamid; Khalil, Hany Ezzat
- Abstract
Natural coumarins are reputed to demonstrate several biological effects to improve adverse health conditions including; obesity. This study was conducted to explore the potential in vitro lipid and glucose modulatory activity of cichorioside on 3T3-L1 cell lines. Methods: The 3T3-L1 cell lines were cultured and cell viability was assessed. Glucose content in medium of cultured cells was measured. Differentiation of 3T3-L1 cells from pre-adipocytes to adipocytes was evaluated upon addition of cichorioside. Expression of mRNA of the peroxisome proliferator-activated receptor gamma (PPARγ) was estimated. Results: Survival of around 90% of cultured cells was observed at 10μM cichorioside. Cichorioside inhibited glucose uptake from the medium by the 3T3-L1 cell lines. Cichorioside considerably inhibited pre-adipocyte differentiation and the lipid content in intercellular storage. Cichorioside demonstrated an upregulation of the mRNA expression of PPARγ. Moreover, the docking studies supported the results via the deep interaction of cichorioside with amino acids residue of PPAR-γ. Taken together, these findings are the first report on in vitro evaluation of cichorioside to modulate the lipid storage and glucose uptake of cultured 3T3-L1 cell lines.
- Subjects
CELL lines; PEROXISOME proliferator-activated receptors; GLUCOSE; AMINO acid residues; CELL culture
- Publication
Pharmacognosy Journal, 2022, Vol 14, p933
- ISSN
0975-3575
- Publication type
Article
- DOI
10.5530/pj.2022.14.192