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- Title
Calcein marking of Hogfish (Lachnolaimus maxi mus), a potential tool for future stock enhancement.
- Authors
Ray, Brandon; Murray, Casey A.; Markham, Olivia; Ohs, Cortney; Patterson, Joshua; Collins, Angela; DiMaggio, Matthew A.
- Abstract
Hogfish (Lachnolaimus maximus), a large wrasse native to the western Atlantic, are a popular sport and food fish whose southern population have been experiencing overfishing for the past 30 years. Despite their popularity, interest in commercial culture has only recently emerged. Poorly defined culture methods persist as an impediment to potential restoration efforts with this species. Additionally, no information currently exists on effective marking procedures for hatchery produced hogfish. Feasibility of culture and potential stock enhancement is predicated on elucidation of effective protocols. To this end, we explored the efficacy of various protocols using Calcein as a marking agent for hogfish. Calcein is a non-lethal, fluorescent, chemical marker that binds to the calcified structures in exposed animals. To assess its potential for use on hogfish, we used the osmotic induction method where animals were exposed to a hypersaline environment for 3.5 minutes prior to immersion in calcein for the same time, thereby resulting in calcein uptake and marking. We tested pre-treatment salinities of 32. 45, 60, and 75 g/L followed by a 3 g/L calcein exposure. A second experiment then tested calcein concentrations of 0, 1, 3, and 5 g/L using the salinity pretreatment that resulted in the brightest mark from the first experiment: only the highest saline and calcein concentrations showed significantly brighter marks than the weakest marked fish. These results identify baseline calcein marking protocols for use with hogfish. Further research into mark retention is warranted to evaluate the usefulness of this tool in potential stock enhancements efforts.
- Subjects
FISH food; FISH as food; WRASSES; OVERFISHING; SALINITY
- Publication
Florida Scientist, 2023, Vol 86, Issue 3, p441
- ISSN
0098-4590
- Publication type
Article