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- Title
SYNBIOTIC EFFECTS OF GALACTO-OLIGOSACCHARIDE, POLYDEXTROSE AND BIFIDOBACTERIUM LACTIS Bi-07 IN VITRO.
- Authors
Mäkeläinen, H.; Ottman, N.; Forssten, S.; Saarinen, M.; Rautonen, N.; Ouwehand, A. C.
- Abstract
Synbiotics are used to manipulate the endogenous microbiota, but whether they are more effective than the constituting pro- and prebiotics alone remains to be determined. The objective of this work was to evaluate galacto-oligosaccharide (GOS), polydextrose (PDX) and Bifidobacterium lactis Bi-07 alone and in combinations in a simulated human colon model. Their effects on the microbial community structure and activity were analysed with flow cytometry, qPCR and chromatographic methods. Probiotic B. lactis was bifidogenic, but had only minor effects on the metabolites produced in the colon model. Prebiotic GOS increased the bifidobacteria, whereas PDX had negative effects on the B. lactis and Clostridium numbers. The prebiotics differed in their effects on the metabolite production. Synbiotic GOS+B lactis increased the levels of Bifidobacterium and decreased the numbers of Lactobacillus, Bacteroides and clostridia, and thus, had more effects on the microbial community structure than the constituting components alone. Also the combination of PDX+GOS+B.lactis had similar beneficial effects on the microbiota and in addition, the two oligosaccharides benefited the production of short-chain fatty acids on the whole length of the colonic model. In this study, the synbiotics including GOS were more effective than the constituting pro- or prebiotics alone in modulating the microbiota composition.
- Subjects
BIFIDOBACTERIUM; PREBIOTICS; FLOW cytometry; FATTY acids; LACTOBACILLUS; POLYDEXTROSE
- Publication
International Journal of Probiotics & Prebiotics, 2010, Vol 5, Issue 4, p203
- ISSN
1555-1431
- Publication type
Article