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- Title
Development and validation of RP- HPLC method with UV detection to determine and quantify dimenhydrinate in human plasma.
- Authors
Zeb-un-nisa; Shoaib, Muhammad Harris; Ali, Syed Imran; Rizvi, Mehwish; Ali, Huma; Fatima, Rasheeda; Khan, Maqsood Ahmed; Kashif, Sadia Suri
- Abstract
A simple, sensitive and rigorous method for estimation of dimenhydrinate in human plasma was searched and its validation was carried out. LLE (Liquid-Liquid extraction) of analyte with mixture of Hexane and ethyl acetate (1:1 v/v) was carried out for the preparation of Plasma Samples, Chromatographic elution of dimenhydrinate was conducted in human plasma and mobile phase with C-18 bonda Pack column (10µm; 250 × 4.6), using a mobile phase consisting a solution of ammonium bicarbonate in water and methanol at a flow rate of 0.5ml/minute with UV detection at 229 nm. The resolution of dimenhydrinate was well performed from plasma components. This method was validated and exhibited linearity with concentration range of 6 to 380ng/ml of dimenhydrinate in plasma. The Intra day precision was 89.2 to 96.89% and Inter day precision was 88.6% to 93.26%, the average recovery of dimenhydrinate was 97.02%. The efficacy of extraction was proved by above mentioned results. 2ng/ml and 6ng/ml, were appraised as the LOD and LOQ of dimenhydrinate, stability studies disclosed that dimenhydrinate exhibited stability in Plasma after Freeze & thaw cycles and upon -20°C storage, the method was developed well.
- Subjects
DIMENHYDRINATE; BLOOD plasma; AMMONIUM bicarbonate; DRUG efficacy; CHROMATOGRAPHIC analysis
- Publication
Pakistan Journal of Pharmaceutical Sciences, 2018, Vol 31, p979
- ISSN
1011-601X
- Publication type
Article