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- Title
Evaluation of environmental conditions for production of bacteriocin-like substance by Bacillus sp. strain P34.
- Authors
Amanda Motta
- Abstract
Abstract The influence of temperature, pH and media on bacteriocin production by Bacillus sp. P34 was investigated. The effect of temperature and initial pH was evaluated by factorial design and response surface methodology (RSM). Statistical analysis of results showed that, in the range studied, the two variables have a significant effect on bacteriocin production. Response-surface data showed maximum antimicrobial activity production at initial pH values between 6.0 and 8.0 and temperatures between 25 and 37 °C. No relationship between bacterial growth and bacteriocin production was observed. RSM proved to be a powerful tool in optimizing the production of antimicrobial activity by Bacillus sp. P34. When different media were tested, maximum bacteriocin production was observed in soybean protein-based medium, but antimicrobial activity was not achieved by cultivation on fish meal, feather meal, whey and grape waste.
- Subjects
MICROBIAL growth; ANAEROBIC bacteria growth; BACILLUS (Bacteria); FACTORIAL experiment designs
- Publication
World Journal of Microbiology & Biotechnology, 2008, Vol 24, Issue 5, p641
- ISSN
0959-3993
- Publication type
Article
- DOI
10.1007/s11274-007-9520-6