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- Title
Reactive Oxygen Species and X-Ray Disrupted Spontaneous [Ca<sup>2+</sup>]<sub>i</sub> Oscillation in Alveolar Macrophages.
- Authors
Chen, Hao; Ye, Hong; Meng, Da-Quan; Cai, Peng-Cheng; Chen, Feng; Zhu, Li-Ping; Tang, Qiu; Long, Zhi-Xiong; Zhou, Qiong; Jin, Yang; Xin, Jian-Bao; Tao, Xiao-Nan; Ma, Wan-Li
- Abstract
Radiation leads to a rapid burst of reactive oxygen species (ROS), which is considered to be one of the major causes of radiation-induced injury. ROS have previously been shown to induce changes in cytosolic Ca2+ ([Ca2+]i) including [Ca2+]i oscillation. However, the role of radiation in [Ca2+]i oscillation is poorly understood. The purpose of this study was to identify the effect of ROS and X ray on [Ca2+]i oscillation, as well as their role in radiation-induced lung injury. Alveolar macrophages were cultured in the absence and presence of different doses of hydrogen peroxide (H2O2) or exposed to X-ray irradiation with or without pretreatment of diphenyleneiodonium chloride (DPI, an inhibitor of NADPH oxidases) or tetrandrine (TET, a calcium entry blocker) and cytosolic Ca2+ concentration was detected by fluorescent Ca2+ indicator Fura-2. Rat radiation lung injury was induced in vivo by using 40 Gy X ray and DPI or TET was used to prevent radiation-induced lung injury. The results showed that there was spontaneous [Ca2+]i oscillation in alveolar macrophages under normal conditions, and treatment of H2O2 (100-500 μ M) or 2 Gy X ray inhibited the spontaneous [Ca2+]i oscillation and induced [Ca2+]i rise. TET abolished H2O2 or X ray induced [Ca2+]i rise in alveolar macrophages, and attenuated X ray- induced rat alveolitis in vivo. DPI prevented X-ray-induced inhibition of [Ca2+]i oscillation in alveolar macrophages and prevented X-ray-induced rat alveolitis. Taken together, the data suggest that the disruption of [Ca2+]i oscillation and induction of [Ca2+]i rise through ROS is involved in the mechanism of radiation-induced lung injury.
- Subjects
REACTIVE oxygen species; ALVEOLAR macrophages; PHYSIOLOGICAL effects of radiation; RADIATION exposure; RADIATION-induced abnormalities; LUNG injuries
- Publication
Radiation Research, 2013, Vol 179, Issue 4, p485
- ISSN
0033-7587
- Publication type
Article
- DOI
10.1667/RR3006.1