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- Title
Increased efficiency of mRNA 3′ end formation: a new genetic mechanism contributing to hereditary thrombophilia.
- Authors
Gehring, Niels H.; Frede, Ute; Neu-Yilik, Gabriele; Hundsdoerfer, Patrick; Vetter, Barbara; Hentze, Matthias W.; Kulozik, Andreas E.
- Abstract
The G→A mutation at position 20210 of the prothrombin or coagulation factor II gene (F2) represents a common genetic risk factor for the occurrence of thromboembolic events. This mutation affects the 3′-terminal nucleotide of the 3′ untranslated region (UTR) of the mRNA and causes elevated prothrombin plasma concentrations by an unknown mechanism. Here, we show that the mutation does not affect the amount of pre-mRNA, the site of 3′ end cleavage or the length of the poly(A) tail of the mature mRNA. Rather, we demonstrate that the physiological F2 3′ end cleavage signal is inefficient and that F2 20210 G→A represents a gain-of-function mutation, causing increased cleavage site recognition, increased 3′ end processing and increased mRNA accumulation and protein synthesis. Enhanced mRNA 3′ end formation efficiency emerges as a novel principle causing a genetic disorder and explains the role of the F2 20210 G→A mutation in the pathogenesis of thrombophilia. This work also illustrates the pathophysiologic importance of quantitatively minor aberrations of RNA metabolism.
- Subjects
PROTHROMBIN; BLOOD coagulation factors; MESSENGER RNA
- Publication
Nature Genetics, 2001, Vol 28, Issue 4, p389
- ISSN
1061-4036
- Publication type
Article
- DOI
10.1038/ng578