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- Title
Identification of Estrogen Response Element in the Aquaporin-2 Gene That Mediates Estrogen-Induced Cell Migration and Invasion in Human Endometrial Carcinoma.
- Authors
Li-Bo Zou; Run-Ju Zhang; Ya-Jing Tan; Guo-Lian Ding; Shuai Shi; Dan Zhang; Rong-Huan He; Ai-Xia Liu; Ting-Ting Wang; Leung, Peter C. K.; Jian-Zhong Sheng; He-Feng Huang
- Abstract
Background: Accumulating evidence suggests that aquaporins (AQP) can facilitate cell migration, invasion, and proliferation in tumor development in addition to water transport. Objective: The aim of this study was to examine AQP2 expression in the endometrial tissues from patients with endometrial carcinoma (EC) and determine the roles and mechanisms of AQP2 in estrogen-related cell migration, invasion, adhesion, and proliferation of Ishikawa (IK) cells. Approach: AQP2 expression levels were measured in human endometrial cells and estradiol (E2)- treated IK cells, and the estrogen-response element was identified. After blocking down and up-regulating the endogenous expression of AQP2 in IK cells, cell morphology, capacity for invasion, migration and adhesion, and expression markers of membrane/cytoskeleton were analyzed. Results: AQP2 was expressed in endometrial tissues from patients with EC and endometriosis, both of which are estrogen-dependent diseases. In IK cells, E2 dose-dependently increased AQP2 expression, which was blocked by the estrogen receptor inhibitor ICI182780. An estrogen-response element was identified in the AQP2 promoter. E2 significantly increased the migration, invasion, adhesion, and proliferation of IK cells. AQP2 knockdown attenuated E2-enhanced migration, invasion, and adhesion. AQP2 knockdown reduced not only the E2-enhanced expression of F-actin and annexin-2 but also the E2-induced alteration of cell morphology. Moreover, higher expression levels of F-actin and annexin-2 were detected in the endometrial tissues from patients with EC. Conclusions: AQP2 mediates E2-enhanced migration, invasion, and adhesion through alteration of F-actin and annexin-2 expression and reorganization of F-actin, and inhibition of AQP may be a potential method for antitumor therapy.
- Publication
Journal of Clinical Endocrinology & Metabolism, 2011, Vol 96, Issue 9, pE1399
- ISSN
0021-972X
- Publication type
Article
- DOI
10.1210/jc.2011-0426