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- Title
Optogenetic control of heart muscle in vitro and in vivo.
- Authors
Bruegmann, Tobias; Malan, Daniela; Hesse, Michael; Beiert, Thomas; Fuegemann, Christopher J.; Fleischmann, Bernd K.; Sasse, Philipp
- Abstract
Electrical stimulation is the standard technique for exploring electrical behavior of heart muscle, but this approach has considerable technical limitations. Here we report expression of the light-activated cation channel channelrhodopsin-2 for light-induced stimulation of heart muscle in vitro and in mice. This method enabled precise localized stimulation and constant prolonged depolarization of cardiomyocytes and cardiac tissue resulting in alterations of pacemaking, Ca2+ homeostasis, electrical coupling and arrhythmogenic spontaneous extrabeats.
- Subjects
ELECTRIC stimulation; MYOCARDIUM; HEART cells; ION channels; HOMEOSTASIS
- Publication
Nature Methods, 2010, Vol 7, Issue 11, p897
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1512