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- Title
MicroRNA-665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10.
- Authors
Liu, Jinhui; Jiang, Yi; Wan, Yicong; Zhou, Shulin; Thapa, Sunita; Cheng, Wenjun
- Abstract
Ovarian cancer is the most lethal gynecological cancer and its metastasis leads to a poor prognosis. The present study was designed to elucidate how microRNA (miR)-665 regulates the proliferation and migration of ovarian tumor cells. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that miR-665 expression was decreased in ovarian cancer tissues. Increased expression of miR-665 suppressed the growth and migration of ovarian cancer cells, whereas the downregulated expression of miR-665 led to the opposite results. Bioinformatics tools identified homeobox A10 (HOXA10) as a target of miR-665. Following miR-665 overexpression, HOXA10 protein expression was significantly reduced. A dual luciferase assay revealed that miR-665 bound to the 3′-untranslated region of HOXA10. Immunohistochemistry and RT-PCR revealed that the expression of HOXA10 was negatively correlated with the expression of miR-665. It was concluded that miR-665 targets HOXA10 and may act as a tumor-suppressing gene in ovarian cancer. This pathway may be involved in the development and metastasis of ovarian cancer.
- Subjects
OVARIAN cancer; OVARIAN diseases; PROGNOSIS; POLYMERASE chain reaction; IMMUNOHISTOCHEMISTRY
- Publication
Molecular Medicine Reports, 2018, Vol 18, Issue 3, p2661
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2018.9252