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- Title
First isolation of SARS-CoV-2 from clinical samples in India.
- Authors
Sarkale, Prasad; Patil, Savita; Yadav, Pragya; Nyayanit, Dimpal; Sapkal, Gajanan; Baradkar, Shrikant; Lakra, Rajen; Shete-Aich, Anita; Prasad, Sharda; Basu, Atanu; Dar, Lalit; Vipat, Veena; Giri, Sidhartha; Potdar, Varsha; Choudhary, Manohar; Praharaj, Ira; Jain, Amita; Malhotra, Bharati; Gawande, Pranita; Kalele, Kaumudi
- Abstract
Sir, The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has, as on March 31, 2020, spread to over 207 countries around the world[[1]],[[2]], with a total of 896,475 confirmed cases and 45,525 deaths[[2]]. Vero CCL-81 cells infected with SARS-CoV-2 strain NIV-2020-770 and uninfected cells (CC) were transferred onto microcavity slides and fixed with acetone. The number of virus copies in the isolates at P-1 in Vero CCL-81 cells ranged from 5.18×10[[7]] to 8.12×10[[8]] copy/ml and increased 1-26 fold to a range of 1.69×10[[8]] to 6.77×10[[9]] in the cell culture supernatants at P-2 [Table 1].{Figure 2}{Table 1} On PID-4, enhanced CPE was observed. This is the first successful virus isolation of SARS-CoV-2 in the Vero CCL-81 cells in India from nasal and throat swabs of persons with a travel history from Italy and their contacts.
- Subjects
INDIA; COVID-19; SARS-CoV-2
- Publication
Indian Journal of Medical Research, 2020, Vol 151, Issue 2, p244
- ISSN
0971-5916
- Publication type
Article
- DOI
10.4103/ijmr.IJMR_1029_20