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- Title
Partial Characterization of Xylanase Produced by Caldicoprobacter algeriensis, a New Thermophilic Anaerobic Bacterium Isolated from an Algerian Hot Spring.
- Authors
Bouacem, Khelifa; Bouanane-Darenfed, Amel; Boucherba, Nawel; Joseph, Manon; Gagaoua, Mohammed; Ben Hania, Wajdi; Kecha, Mouloud; Benallaoua, Said; Hacène, Hocine; Ollivier, Bernard; Fardeau, Marie-Laure
- Abstract
To date, xylanases have expanded their use in many processing industries, such as pulp, paper, food, and textile. This study aimed the production and partial characterization of a thermostable xylanase from a novel thermophilic anaerobic bacterium Caldicoprobacter algeriensis strain TH7C1 isolated from a northeast hot spring in Algeria. The obtained results showed that C. algeriensis xylanase seems not to be correlated with the biomass growth profile whereas the maximum enzyme production (140.0 U/ml) was recorded in stationary phase (18 h). The temperature and pH for optimal activities were 70 °C and 11.0, respectively. The enzyme was found to be stable at 50, 60, 70, and 80 °C, with a half-life of 10, 9, 8, and 4 h, respectively. Influence of metal ions on enzyme activity revealed that Ca enhances greatly the relative activity to 151.3 %; whereas Hg inhibited significantly the enzyme. At the best of our knowledge, this is the first report on the production of xylanase by the thermophilic bacterium C. algeriensis. This thermo- and alkaline-tolerant xylanase could be used in pulp bleaching process.
- Subjects
ALGERIA; XYLANASE biotechnology; HOT springs; ISOLATION of biotechnological microorganisms; THERMOPHILIC bacteria; ANAEROBIC bacteria growth
- Publication
Applied Biochemistry & Biotechnology, 2014, Vol 174, Issue 5, p1969
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-014-1153-2