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- Title
Effects of In Vitro Simulated Gastrointestinal Digestion on the α-Glucosidase Inhibitory Activities of Thai folk Remedies: Synergistic Effects with Acarbose.
- Authors
Damsud, Thanakorn; Ratmanee, Pennapa; Aekkalakburut, Suntaree; Saenjum, Chalermpong; Lila, Mary Ann
- Abstract
α-Glucosidase, an enzyme that plays a crucial role in glucose absorption in the intestine, is a key therapeutic intervention in the management of diabetes mellitus. The inhibition of this enzyme is critical for controlling postprandial hyperglycemia. The present study aims to investigate the stability of phenolic content, total flavonoid content, and α-glucosidase inhibition activities of six Thai folk anti-diabetes remedies by in vitro simulated gastrointestinal digestion methods, as well as to evaluate their synergistic effect with acarbose. The TFD-04 decoction and 90% EtOH extract showed higher total phenolic and flavonoid content (86.83 mg GAE/g extract, 10.00 mg QE/g extract) and (75.32 mg GAE/g extract, 12.45 mg QE/g extract), respectively. The α- glucosidase inhibitory activities (maltase and sucrase) of TFD-04 were found maximum in decoction and GD-decoction with IC50 values of 0.25±0.02 and 0.78±0.04 mg/mL, respectively, when compared with acarbose with statistical significance (P< 0.05). The IC50 values of 0.59±0.02 and 1.59±0.02 mg/mL, respectively. In addition, the decoction extract of TFD-04 showed the most potent synergistic inhibitory effect with acarbose. A kinetic analysis showed that TFD-04 showed uncompetitive inhibition against intestinal maltase. This study suggests that decoction extracts of TFD-04 may serve as effective anti-hyperglycemic remedies in the field of diabetic therapy.
- Subjects
GLUCOSIDASES; TREATMENT of diabetes; HYPOGLYCEMIC agents; HYPERGLYCEMIA; FLAVONOIDS
- Publication
Tropical Journal of Natural Product Research, 2023, Vol 7, Issue 12, p5548
- ISSN
2616-0684
- Publication type
Article
- DOI
10.26538/tjnpr/v7i12.26