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- Title
Quercetin alleviates neonatal hypoxic-ischemic brain injury by inhibiting microglia-derived oxidative stress and TLR4-mediated inflammation.
- Authors
Le, Kai; Song, Zhiping; Deng, Jie; Peng, Xin; Zhang, Jun; Wang, Liang; Zhou, Lu; Bi, Haidi; Liao, Zhengyu; Feng, Zhen
- Abstract
Objective and design: Microglia stimulated by oxygen glucose deprivation (OGD) were treated with quercetin to investigate the effect on oxidative stress and the inflammatory response and to explore whether toll-like receptor 4 (TLR4) signaling was involved. In addition, the effect of quercetin on the neurological functions of neonatal mice with hypoxic-ischemic brain injury (HIBI) was examined. Materials and subjects: Mouse BV2 microglial cells and postnatal day 7 neonatal mice were used. Treatment: A predetermined concentration of quercetin was used in cell experiments. Quercetin was injected i.p. (50 mg/kg) at three time points after HI insult: 0, 24, and 48 h. Methods: Cell viability assay, Western blotting, qRT-RCR, ELISA, HIBI model construction and behavioral tests. Results: This study first showed that quercetin protected BV2 cells from OGD-induced damage and reversed the changes in microglial oxidative stress-related molecules. Second, quercetin inhibited OGD-induced expression of inflammatory factors in BV2 cells and suppressed TLR4/MyD88/NF-κB signaling. Finally, quercetin was disclosed to be effective in mitigating cerebral infarct volume and cognitive and motor function deficits in HIBI mice. Conclusion: These results suggest that the neuroprotective effect of quercetin in HIBI mice is partially due to the inhibition of oxidative stress and TLR4-mediated inflammatory responses in activated microglia.
- Subjects
QUERCETIN; OXIDATIVE stress; BRAIN injuries; INFLAMMATION; CEREBRAL infarction; TOLL-like receptors
- Publication
Inflammation Research, 2020, Vol 69, Issue 12, p1201
- ISSN
1023-3830
- Publication type
Article
- DOI
10.1007/s00011-020-01402-5