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- Title
Interactive biocatalysis achieved by driving enzyme cascades inside a porous conducting material.
- Authors
Siritanaratkul, Bhavin; Megarity, Clare F.; Herold, Ryan A.; Armstrong, Fraser A.
- Abstract
An emerging concept and platform, the electrochemical Leaf (e-Leaf), offers a radical change in the way tandem (multi-step) catalysis by enzyme cascades is studied and exploited. The various enzymes are loaded into an electronically conducting porous material composed of metallic oxide nanoparticles, where they achieve high concentration and crowding – in the latter respect the environment resembles that found in living cells. By exploiting efficient electron tunneling between the nanoparticles and one of the enzymes, the e-Leaf enables the user to interact directly with complex networks, rendering simultaneous the abilities to energise, control and observe catalysis. Because dispersion of intermediates is physically suppressed, the output of the cascade – the rate of flow of chemical steps and information – is delivered in real time as electrical current. Myriad enzymes of all major classes now become effectively electroactive in a technology that offers scalability between micro-(analytical, multiplex) and macro-(synthesis) levels. This Perspective describes how the e-Leaf was discovered, the steps in its development so far, and the outlook for future research and applications. The electrochemical Leaf (e-Leaf) is an emerging technology that addresses complex enzyme cascades nanoconfined within a porous conducting material—exploiting efficient electron tunneling and local NADP(H) recycling to transduce catalysis and electricity. Here, the authors describe how the e-Leaf was discovered, the steps in its development so far, and the outlook for future research and applications.
- Subjects
POROUS materials; MULTIENZYME complexes; ELECTRON tunneling; TECHNOLOGICAL innovations; ENZYMES; BIOCATALYSIS; MESOPOROUS materials; QUANTUM tunneling composites
- Publication
Communications Chemistry, 2024, Vol 7, Issue 1, p1
- ISSN
2399-3669
- Publication type
Article
- DOI
10.1038/s42004-024-01211-5