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- Title
Improvements of Fertility Restoration in Cytoplasmic Male Sterile Cotton by Enhanced Expression of Glutathione S-Transferase (GST) Gene.
- Authors
Bibi, Noreen; Yuan, Shuna; Zhu, Yunguo; Wang, Xuede
- Abstract
The molecular and biochemical bases of fertility restoration were explored using cytoplasmic male sterile (CMS) Ji A, maintainer Ji B, and two cotton hybrids RF and QF, developed by crossing CMS with DES-HAF277 (normal restorer) and Zheda strong restorer (transgenic restorer with GST gene), respectively. Transcript levels of both exogenous and endogenous GST genes were high in anther as compared to other plant tissues of the QF hybrid. Moreover, the expression of the GST gene during meiosis (stage 2) and microspore development (stage 3) was highest in the QF hybrid. The ratio of cyanide-resistant respiration to total respiration was also high in the QF hybrid during stage 2 and stage 3 as compared to the RF hybrid. O and HO contents increased more during stage 2 in the CMS line and stage 3 in the RF hybrid compared to the maintainer and QF hybrid. Similarly, MDA contents were at a maximum in CMS followed by the RF, QF, and the maintainer line during the whole course of anther development. In addition, the activities of antioxidant enzymes (SOD, POD, CAT, APX, GST, GR, and DHAR) and contents of non-enzymatic antioxidants (GSH and ASA) were elevated in the QF hybrid as compared to the RF hybrid, during the whole course of anther development. The present study suggests that the introgression of the GST gene into restorer lines could be a potential way to enhance restoration capability by maintaining the equilibrium between oxidative stress and scavenging enzymes, and might favor healthier development of microspores.
- Subjects
PLANT fertility; CYTOPLASMIC male sterility; GLUTATHIONE transferase; GENE expression in plants; CYANIDES; COTTON; PLANT cells &; tissues
- Publication
Journal of Plant Growth Regulation, 2014, Vol 33, Issue 2, p420
- ISSN
0721-7595
- Publication type
Article
- DOI
10.1007/s00344-013-9393-x