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- Title
Next-generation sequencing: Pyrosequencing and its utilization in medical field.
- Authors
Pal, Subhajit; Sarangi, Suman; Paul, Santanu
- Abstract
Next-generation sequencing (NGS) can be an idle innovation utilized for DNA and RNA sequencing and variant or mutation study. NGS can arrangement huge qualities or entire genome in a brief period of time. The arrangement variants or mutations recognized by NGS have been broadly utilized for infection. DNA sequencing is one of the foremost critical stages for consideration of organic frameworks nowadays. Arrangement assurance is most commonly performed utilizing deoxy chain end innovation. As of late, pyrosequencing has developed as an unused sequencing methodology. This strategy could be a broadly appropriate, elective innovation for the point by point characterization of nucleic acids. Pyrosequencing has the potential focal points of exactness, adaptability, parallel preparing, and can be effortlessly mechanized. Besides, the strategy apportions with the requirement for abled groundworks, labeled nucleotides, and gelelectrophoresis. This article considers key highlights with respect to distinctive viewpoints of pyrosequencing innovation, counting the common standards, chemical properties, sequencing modes, instrumented, and potential applications. By using these techniques, the DNA sequencing subsequently the precision medicinal field can be explore for the therapeutics approaches for different diseases.
- Subjects
PYROSEQUENCING; NUCLEIC acids; DNA sequencing; RNA sequencing; NUCLEOTIDE sequencing; CHEMICAL properties
- Publication
Journal of Advanced Zoology, 2023, Vol 44, p2033
- ISSN
0253-7214
- Publication type
Article
- DOI
10.17762/jaz.v44is6.2690