We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Quantitative Proteomic Analysis Deciphers the Molecular Mechanism for Endosperm Nuclear Division in Early Rice Seed Development.
- Authors
Yoon, Jinmi; Min, Cheol Woo; Kim, Jiyoung; Baek, Gibeom; Kim, Dohyeon; Jang, Jeong Woo; Gupta, Ravi; Kim, Sun Tae; Cho, Lae-Hyeon
- Abstract
Understanding the molecular mechanisms underlying early seed development is important in improving the grain yield and quality of crop plants. We performed a comparative label-free quantitative proteomic analysis of developing rice seeds for the WT and osctps1-2 mutant, encoding a cytidine triphosphate synthase previously reported as the endospermless 2 (enl2) mutant in rice, harvested at 0 and 1 d after pollination (DAP) to understand the molecular mechanism of early seed development. In total, 5231 proteins were identified, of which 902 changed in abundance between 0 and 1 DAP seeds. Proteins that preferentially accumulated at 1 DAP were involved in DNA replication and pyrimidine biosynthetic pathways. Notably, an increased abundance of OsCTPS1 was observed at 1 DAP; however, no such changes were observed at the transcriptional level. We further observed that the inhibition of phosphorylation increased the stability of this protein. Furthermore, in osctps1-2, minichromosome maintenance (MCM) proteins were significantly reduced compared with those in the WT at 1 DAP, and mutations in OsMCM5 caused defects in seed development. These results highlight the molecular mechanisms underlying early seed development in rice at the post-transcriptional level.
- Subjects
SEED development; RICE seeds; ENDOSPERM; CROPS; CROP quality
- Publication
Plants (2223-7747), 2023, Vol 12, Issue 21, p3715
- ISSN
2223-7747
- Publication type
Article
- DOI
10.3390/plants12213715