The possible application of Rhоdococcus qingshengii strain GlMm1, isolated from a Dead Sea clay sample, as the basis of a biosensor for determining the benzimidazole fungicide carbendazim was investigated. High sensitivity of the biosensor under neutral pH and up to 500 mM NaCl at 2 to 160 μM carbendazim was maintained for up to 30 days.