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- Title
Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury.
- Authors
Costa Rodrigues Pinheiro, Aruanã Joaquim Matheus; Sá Gonçalves, Jaciara; Alves Dourado, Ádylla Wilenna; Martins de Sousa, Eduardo; Mesquita Brito, Natilene; Silva, Lanna Karinny; Aranha Batista, Marisa Cristina; Cortez de Sá, Joicy; Aragão Vieira Monteiro, Cinara Regina; Fernandes, Elizabeth Soares; Monteiro-Neto, Valério; Campbell, Lee Ann; Wiziack Zago, Patrícia Maria; Gonçalves Lima-Neto, Lidio
- Abstract
The hydroalcoholic extract of Punica granatum (pomegranate) leaves was previously demonstrated to be anti-inflammatory in a rat model of lipopolysaccharide- (LPS-) induced acute peritonitis. Here, we investigated the anti-inflammatory effects of the ethyl acetate fraction obtained from the pomegranate leaf hydroalcoholic extract (EAFPg) on the LPS-induced acute lung injury (ALI) mouse model. Male Swiss mice received either EAFPg at different doses or dexamethasone (per os) prior to LPS intranasal instillation. Vehicle-treated mice were used as controls. Animals were culled at 4 h after LPS challenge, and the bronchoalveolar lavage fluid (BALF) and lung samples were collected for analysis. EAFPg and kaempferol effects on NO and cytokine production by LPS-stimulated RAW 264.7 macrophages were also investigated. Pretreatment with EAFPg (100–300 mg/kg) markedly reduced cell accumulation (specially neutrophils) and collagen deposition in the lungs of ALI mice. The same animals presented with reduced lung and BALF TNF-α and IL-1β expression in comparison with vehicle controls (p < 0 05). Additionally, incubation with either EAFPg or kaempferol (100 μg/ml) reduced NO production and cytokine gene expression in cultured LPS-treated RAW 264.7 macrophages. Overall, these results demonstrate that the prophylactic treatment with EAFPg attenuates acute lung inflammation. We suggest this fraction may be useful in treating ALI.
- Publication
Journal of Immunology Research, 2018, p1
- ISSN
2314-8861
- Publication type
Article
- DOI
10.1155/2018/6879183