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- Title
Molecular-level effects of acupuncture on depression: a genome-wide transcriptome analysis of pituitary gland in rats exposed to chronic restraint stress.
- Authors
Wang Yu; Jiang Huili; Meng Hong; Li Jing; Yang Xinjing; Zhao Bingcong; Yu Qiuyun; Liang Xingchen; Bao Tuya
- Abstract
OBJECTIVE: To investigate the antidepressant mechanism of acupuncture at the molecular level in the pituitary gland of rats exposed to chronic restraint stress (CRS). METHODS: Forty-eight rats were randomly divided into a control group (C) model group (M) fluoxetine group (F) and acupuncture group (A). CRS was induced in the model group by restraining rats for 28 days. Groups F and A were given fluoxetine administration and acupuncture intervention respectively 1 h before the CRS procedures were implemented. On the 29th day rat pituitary glands were harvested for RNA extraction and RNA-sequencing analysis. RESULTS: Differentially expressed gene analysis identified 148 87 and 179 response genes differentially expressed in group C vs group M group F vs group M and group A vs group M respectively. Using Gene Ontology term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis we identified the up-regulation of gene sets involved in extracellular space receptor binding and monoamine neurotransmitter metabolism and the down-regulation of gene sets involved in immune response and inflammatory response as the prevailing transcriptomic signatures in the pituitary gland of rats treated with CRS fluoxetine or acupuncture. CONCLUSION: Our findings suggest that acupuncture has a multitarget antidepressant effect at the molecular and behavioral levels.
- Publication
Journal of Traditional Chinese Medicine, 2017, Vol 37, Issue 4, p486
- ISSN
0255-2922
- Publication type
Article
- DOI
10.1016/s0254-6272(17)30155-3