We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Hsk1-Dfp1 is required for heterochromatin-mediated cohesion at centromeres.
- Authors
Bailis, Julie M.; Bernard, Pascal; Antonelli, Richard; Allshire, Robin C.; Forsburg, Susan L.
- Abstract
Heterochromatin performs a central role in chromosome segregation and stability by promoting cohesion at centromeres. Establishment of both heterochromatin-mediated silencing and cohesion requires passage through S phase, although the mechanism is unknown. Here we demonstrate that Schizosaccharomyces pombe Hsk1 (CDC7), a conserved Dbf4-dependent protein kinase (DDK) that regulates replication initiation, interacts with and phosphorylates the heterochromatin protein 1 (HP1) equivalent Swi6 (ref. 6). Hsk1 and its regulatory subunit Dfp1 function downstream of Swi6 localization to promote heterochromatin function and cohesion specifically at centromeres. This role for Hsk1-Dfp1 is separable from its replication initiation activity, providing a temporal link between S phase and centromere cohesion that is mediated by heterochromatin.
- Subjects
CHROMOSOME analysis; HETEROCHROMATIN; CHROMATIN; COHESION; PHOSPHORYLATION; CENTROMERE
- Publication
Nature Cell Biology, 2003, Vol 5, Issue 12, p1111
- ISSN
1465-7392
- Publication type
Article
- DOI
10.1038/ncb1069