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- Title
Smooth-muscle contraction without smooth-muscle myosin.
- Authors
Morano, Ingo; Chai, Gui-Xuan; Baltas, Leonidas G.; Lamounier-Zepter, Valeria; Lutsch, Gudrun; Kott, Monika; Haase, Hannelore; Bader, Michael
- Abstract
Here we have used gene-targeting to eliminate expression of smooth-muscle myosin heavy chain. Elimination of this gene does not affect expression of non-muscle myosin heavy chain, and knockout individuals typically survive for three days. Prolonged activation, by KCI depolarisation, of intact bladder preparations from wild-type neonatal mice produces an initial transient state (phase 1) of high force generation and maximal shortening velocity, which is followed by a sustained state (phase 2) characterized by low force generation and maximal shortening velocity. Similar preparations from knockout neonatal mice do not undergo phase 1, but exhibit a normal phase 2. We propose that, in neonatal smooth muscle phase 1 is generated by recruitment of smooth-muscle myosin heavy chain, whereas phase 2 can be generated by activation of non-muscle myosin heavy chain. We conclude that phase I becomes indispensable for survival and normal growth soon after birth, particularly for functions such as homeostasis and circulation.
- Subjects
GENE targeting; MYOSIN; SMOOTH muscle
- Publication
Nature Cell Biology, 2000, Vol 2, Issue 6, p371
- ISSN
1465-7392
- Publication type
Article
- DOI
10.1038/35014065