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- Title
Fabrication of SrTiO<sub>3</sub> Layer on Pt Electrode for Label-Free Capacitive Biosensors.
- Authors
Malvano, Francesca; Maritato, Luigi; Carapella, Giovanni; Orgiani, Pasquale; Pilloton, Roberto; Di Matteo, Marisa; Albanese, Donatella
- Abstract
Due to their interesting ferroelectric, conductive and dielectric properties, in recent years, perovskite-structured materials have begun to attract increasing interest in the biosensing field. In this study, a strontium titanate perovskite layer (SrTiO3) has been synthesized on a platinum electrode and exploited for the development of an impedimetric label-free immunosensor for Escherichia coli O157:H7 detection. The electrochemical characterization of the perovskite-modified electrode during the construction of the immunosensor, as well as after the interaction with different E. coli O157:H7 concentrations, showed a reproducible decrease of the total capacitance of the system that was used for the analytical characterization of the immunosensor. Under optimized conditions, the capacitive immunosensor showed a linear relationship from to 1 to 7 log cfu/mL with a low detection limit of 1 log cfu/mL. Moreover, the atomic force microscopy (AFM) technique underlined the increase in roughness of the SrTiO3-modified electrode surface after antibody immobilization, as well as the effective presence of cells with the typical size of E. coli.
- Subjects
BIOSENSORS; PEROVSKITE; STRONTIUM titanate
- Publication
Biosensors (2079-6374), 2018, Vol 8, Issue 1, p26
- ISSN
2079-6374
- Publication type
Article
- DOI
10.3390/bios8010026