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- Title
Efectos agudos de fenantreno y benzo[a]pireno en Aequidens metae (Pisces: Cichlidae).
- Authors
María Velasco-Santamaría, Yohana; Cardenas-Camacho, Jessica; Mauricio Peñuela-Díaz, Manuel; Alexander Jaramillo-Hernández, Dumar; Corredor-Santamaría, Wilson
- Abstract
Aquatic organisms may be exposed to industrial effluents that often contain polycyclic aromatic hydrocarbons (PAHs). Many of their effects may go unnoticed, therefore, the assessment of biomarkers allows the evaluation of the risks that may generate health damage associated with their metabolism. Different biomarkers were evaluated in Aequidens metae exposed to PAHs. Adults of 6,3 ± 1,2 cm and 5,5 ± 1,6 g were used. Fish were exposed intraperitoneally to 1, 10 and 50 µg/g phenanthrene (PHE), 0,1, 1; and 10 μg/g benzo[a]Pyrene (B[a]P) and 50 µg/g ß-naphthoflavone. Sampling was performed at 0, 3 and 10 days. Liver and peripheral blood samples were taken for processing. 7-ethoxyresorufin-O-deethylase (EROD) activity, hepatocyte histometry and frequency of micronuclei and other abnormalities in blood were evaluated. We found an increase in EROD activity at day 3 of exposure for B[a]P of 10 µg/g (11,82 ± 2,27 pmol/mg/min) and BNF (9,11 ± 3,04 pmol/mg/min) in relation to the negative control (p<0.05). Also, tissue and genotoxic response was presented with PHE, B[a]P and BNF at medium and high concentrations. We can conclude that, under the conditions of this study, B[a]P is a strong inducer that activates the CYP1A biotransformation pathway compared to PHE, furthermore, EROD activity, nuclear abnormalities and histometric characteristics of hepatocytes dependent on exposure time, PAH concentration and the PAH characteristics in A. metae.
- Subjects
POLYCYCLIC aromatic hydrocarbons; INDUSTRIAL wastes; PYRENE; PHENANTHRENE; BIOMARKERS
- Publication
Revista Colombiana de Biotecnología, 2024, Vol 26, Issue 1, p42
- ISSN
0123-3475
- Publication type
Article
- DOI
10.15446/rev.colomb.biote.v26n1.101879