We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Polymerase chain reaction detection of Bartonella henselae bacteraemia in an immunocompetent child with cat-scratch disease.
- Authors
Del Prete, Raffaele; Fumarola, Donato; Ungari, Sandro; Fumarola, Luciana; Miragliotta, Giuseppe; Del Prete, R; Fumarola, D; Ungari, S; Fumarola, L; Miragliotta, G
- Abstract
<bold>Unlabelled: </bold>A case of Bartonella henselae bacteraemia is reported in an immunocompetent 8-year-old boy with cat-scratch disease. Serology to B. henselae, diagnosed by polymerase chain reaction, was positive. DNA was extracted from peripheral whole blood and amplified with specific primers targeting the htrA gene of B. henselae. A non-isotopic hybridization assay with a species-specific oligonucleotide probe was used to detect the amplified product.<bold>Conclusion: </bold>The polymerase chain reaction can be used for the rapid laboratory diagnosis of bacteraemia in cat-scratch disease.
- Subjects
BARTONELLA infections; CAT-scratch disease; POLYMERASE chain reaction; NUCLEIC acid hybridization; NUCLEIC acid probes; BACTEREMIA diagnosis; DNA analysis; COMPARATIVE studies; FLUORESCENT antibody technique; GRAM-negative bacteria; GRAM-negative bacterial diseases; RESEARCH methodology; MEDICAL cooperation; RESEARCH; EVALUATION research; BACTERIAL antibodies
- Publication
European Journal of Pediatrics, 2000, Vol 159, Issue 5, p356
- ISSN
0340-6199
- Publication type
journal article
- DOI
10.1007/s004310051286