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- Title
Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis.
- Authors
Salem, Maryam; Khadivi, Farnaz; Javanbakht, Parinaz; Mojaverrostami, Sina; Abbasi, Mehdi; Feizollahi, Narjes; Abbasi, Yasaman; Heidarian, Ehsan; Rezaei Yazdi, Farzane
- Abstract
The loss of germ cells and spermatogenic failure in non-obstructive azoospermia are believed to be the main causes of male infertility. Laboratory studies have used in vitro testicular models and different 3-dimensional (3D) culture systems for preservation, proliferation and differentiation of spermatogonial stem cells (SSCs) in recent decades. The establishment of testis-like structures would facilitate the study of drug and toxicity screening, pathological mechanisms and in vitro differentiation of SSCs which resulted in possible treatment of male infertility. The different culture systems using cellular aggregation with self-assembling capability, the use of different natural and synthetic biomaterials and various methods for scaffold fabrication provided a suitable 3D niche for testicular cells development. Recently, 3D culture models have noticeably used in research for their architectural and functional similarities to native microenvironment. In this review article, we briefly investigated the recent 3D culture systems that provided a suitable platform for male fertility preservation through organ culture of testis fragments, proliferation and differentiation of SSCs.
- Subjects
CELL culture; MALE infertility; FERTILITY preservation; DRUG toxicity; ORGAN culture; SPERMATOGENESIS; AZOOSPERMIA; GONADS
- Publication
Stem Cell Research & Therapy, 2023, Vol 14, Issue 1, p1
- ISSN
1757-6512
- Publication type
Article
- DOI
10.1186/s13287-023-03466-6