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- Title
Secretion of Gut Hormones and Expression of Sweet Taste Receptors and Glucose Transporters in a Rat Model of Obesity.
- Authors
Feng, Ri Lu; Qian, Cheng; Liu, Lian Yong; Liu, Qian Jing; Jin, Yun Qiu; Li, Sheng Xian; Liu, Wei; Rayner, Chris K.; Ma, Jing
- Abstract
Objectives: This study was undertaken to compare gut hormone secretion between high-fat-fed and control rats, and to examine the corresponding changes in the expression of sweet taste receptors and glucose transporters in the small intestine and hypothalamus. Methods: Four-week-old male Sprague Dawley rats were fed a standard or high-fat diet for 8 weeks (10 in each group), followed by an oral glucose tolerance test (50% glucose solution, 2 g/kg). Blood was sampled for glucose, insulin, glucagon-like peptide-1 (GLP-1) and polypeptide YY (PYY) assays. One week later, small intestinal and hypothalamic tissue were analyzed for sweet taste receptor and glucose transporter expression by real-time PCR. Results: After oral glucose, plasma GLP-1 concentrations were higher in high-fat-fed than standard-fat-fed rats (group × time interaction, p < 0.01) with significant differences at t = 15 min (p < 0.01) and 30 min (p < 0.05). Plasma PYY concentrations were lower in high-fat-fed than control rats at t = 0, 15 min (p < 0.05, respectively) and 120 min (p < 0.01). There were no differences in the expression of sweet taste receptors or glucose transporters between high-fat-fed and control rats in the duodenum, ileum, or hypothalamus. Conclusions: Changes in GLP-1 and PYY secretion after a high-fat diet appear unrelated to any changes in the expression of sweet taste receptors or glucose transporters. Impaired PYY secretion with high-fat feeding suggests that PYY analogues may provide a potential therapy in the treatment of obesity.
- Subjects
TASTE receptors; SWEETNESS (Taste); GASTROINTESTINAL hormones; SECRETION; GASTRIC inhibitory polypeptide; SPRAGUE Dawley rats; GLUCOSE transporters
- Publication
Obesity Facts: The European Journal of Obesity, 2019, Vol 12, Issue 2, p190
- ISSN
1662-4025
- Publication type
Article
- DOI
10.1159/000497122