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- Title
Factores histológicos relacionados con resultados discordantes entre la biopsia y la prueba de ADN, en el diagnóstico del virus papiloma humano (VPH).
- Authors
Toro, Rubén; Reigosa, Aldo; Castro, Julio; Contreras, Adriana; Del Villar, María; González, Pamela; Álvarez, Maritza
- Abstract
The diagnosis of cervical infection caused by human papilloma virus (HPV) is made basically through the biopsy and/or molecular methods such as polymerase chain reaction (PCR). The results of these studies sometimes do not match. In this work is evaluated the possible influence of different factors associated with results false positive and false negative of both tests. Were analyzed 271 samples from uterin cervix of patients presenting to the gynecologist during the period 1999-2009, to which were taken biopsies due present findings colposcopics and/or cytological suspicious of HPV infection. Considering the result of PCR as gold standard test, biopsy showed 14% false positive and false negative 16.6%, the diagnostic of VPH in biopsy and PCR showed greater concordance when samples include endo-exocervical transformation zone, and the presence of squamous metaplasia in the sample and size of the largest fragment (>0.5 cm); while the fixation time, the type and intensity of inflammation, bleeding and erosion of the epithelium were not significant. In conclusion, in order to reduce the discrepancy between the results of the biopsy and test DNA, it is important that the biopsy sample includes the transformation zone of the uterin cervix and at least one fragment greater than 0.5 cm.
- Subjects
CERVIX uteri disease diagnosis; PAPILLOMAVIRUS disease diagnosis; VIRAL disease diagnosis; DIAGNOSTIC use of polymerase chain reaction; BIOPSY; METAPLASIA
- Publication
Salus Online, 2016, Vol 20, Issue 2, p30
- ISSN
1316-7138
- Publication type
Article