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- Title
Correlation of the Probiotic Cell Number ml<sup>-1</sup> and the Cell Mediated Immune Response: an in vitro Study.
- Authors
BHATIA, ARUNA; RANDHAWA, MANSIMRAN KAUR
- Abstract
Probiotics are live microorganisms which when administered in adequate amount confer a health benefit on the host. The beneficial effects of probiotics have been related to their survival number in the gut. Moreover, different strains differ with regard to their ability to colonize and proliferate in the GI tract due to which the efficiency of any therapeutic effect would also be expected to be strain dependant. It is expected that the minimum concentration of a probiotic microorganisms required demonstrating health promoting effects will also be dependent on many factors. Hence, this study was planned to evaluate the in vitro, cell mediated immune response of 6 probiotic strains by differing their cell number ml-1 viz. 1 × 106 cell ml-1 and 1 × 109 cell ml-1 by using pig splenocytes. Splenocytes which were incubated with the six strains separately at concentrations of 1 × 106 cell ml-1 and 1 × 109 cell ml-1 for 24 hours, were assayed to study the cell mediated immune response by employing the Nitroblue Tetrazolium Reduction test, the Inducible Nitric Oxide Synthase test and by studying the bactericidal activity. The results demonstrated that a substantial increase in the stimulation of the cells occurred by the effect of all the probiotic strains at both the concentrations i.e., 1 × 106 cell ml-1 and 1 × 109 cell ml-1.However, the stimulation of the splenocytes was invariably higher at the concentration of 1 × 109 cell ml-1. The study suggests that higher number of cells should be employed during animal experimentation with probiotics to get observable effects.
- Subjects
PROBIOTICS; MICROORGANISMS; IMMUNE response; NITROBLUE tetrazolium; NITRIC oxide
- Publication
Journal of Clinical & Diagnostic Research, 2012, Vol 6, Issue 5, p825
- ISSN
0973-709X
- Publication type
Article