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- Title
Industrial Bioprocessing Strategies for Cultivation of Local Streptomyces violaceoruber Strain SYA3 to Fabricate Nano-ZnO as Anti-Phytopathogens Agent.
- Authors
EL-Moslamy, Shahira H.; El-Morsy, El-Sayed M.; Mohaisen, Marwa T.; Rezk, Ahmed H.; Abdel-Fattah, Yasser R.
- Abstract
Recently, applying industrial bio-processing strategies are being a promised tool that used to produce large quantities of bioactive metabolites from different microbial cells. So in this study, the statistical experimental designs (Plackett-Burman and Box-Behnken) were used for optimization of local S. violaceoruber SYA3 biomass production (18g/l) and fabrication the highest nano-ZnO mass weight (15g/l). Since the nano-ZnO was fabricated by using the extracted periplasmic metabolites as a reducing agent and Zn(NO3)2.6 H2O as a precursor. The morphological properties and size of the nano-ZnO were evaluated using SEM and TEM analysis. Large-scale production was studied via batch fermentation mode using semi-industrial scale bioreactor (8.9L). The biomass production and the fabricated nano-ZnO was (22.8g/l) and (56.8g/l), respectively. Finally, this work reported developing a new cheap and eco-friendly nano-ZnO fabrication method and applying nano-ZnO as a strong anti-phytopathogens agent.
- Subjects
STREPTOMYCES; BIOSYNTHESIS; ZINC oxide; BIOMASS; SCANNING electron microscopes
- Publication
Journal of Pure & Applied Microbiology, 2018, Vol 12, Issue 3, p1133
- ISSN
0973-7510
- Publication type
Article
- DOI
10.22207/JPAM.12.3.12