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- Title
Phage display-derived immunorecognition elements LSPR nanobiosensor for peptide hormone glycine-extended gastrin 17 detection.
- Authors
Fattahi, Zahra; Tohidkia, Mohammad Reza; Yari Khosroushahi, Ahmad
- Abstract
The current study intended to evaluate two types of biorecognition element (BRE), namely recombinant antibody fragments and M13 bacteriophage-displayed antibody fragments, where protein L and electrostatic interactions were used to respectively conjugated antibodies and bacteriophages on AuNPs. The functionalization process was examined by DLS to monitor the changes in the size and zeta potential. The formation of the BRE-G17-Gly immunological complexes was manifested by aggregation (confirmed by FE-SEM) and color change from red to dark blue visible to the naked eye. Local refractive index variations of functionalized AuNPs were monitored by a UV − vis spectrophotometer, showing increasing size and decreasing zeta potential in all stages. The calibration plot was developed in the concentration range 1–5 µg/mL and the limit of detection (LOD) was 1 µg/mL. The LSRP nanobiosensor in combination with the phage-based BRE was an affordable and simple approach, as it was able to eliminate the time-consuming and costly step of extracting antibodies. Contrary to the traditional immunoassays, this method does not require additional amplification, e.g., enzymatic, to read the result. The proposed LSPR nanobiosensor model can be adapted to detect a wide range of pathogens, viruses, and biomarkers in the shortest possible time.
- Subjects
PEPTIDE hormones; BACTERIOPHAGES; IMMUNE recognition; RECOMBINANT antibodies; SURFACE plasmon resonance; ZETA potential
- Publication
Microchimica Acta, 2022, Vol 189, Issue 1, p1
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-021-05159-4